Total T3 ELISA Kit

Total T3 ELISA Kit Specification

Control Type
Manual

Accuracy
High Sensitivity and Specificity

Features
Ready-to-use reagents, Sealed Breakable Microplate Strips, High Sensitivity

Shape
Rectangular (Microplate)

Type
ELISA Kit

Equipment Type
Immunoassay Kit

Equipment Materials
Plastic, Polystyrene Microplate

Power
Not Required (Manual)

Material
High Grade Reagents, Polystyrene

Application
Quantitative Detection of Total T3 in Human Serum or Plasma

Total T3 ELISA Kit Trade Information

Minimum Order Quantity
2 kits Kits

Main Domestic Market
All India

About Total T3 ELISA Kit

T3 totalis a one-step immunoasay to determine the presence of total triiodothyronine (total T3) in human serum using competitive micro plate enzymeimmunoassay.Platesare coated with anti-T3 antibodies.Serum reference, patient specimens or control serum is first added tomicroplate well. Enzyme-T3 conjugate is added. A competition reaction results between the enzyme-triiodothyronine conjugate and a serum containing the nativetotal triiodothyronine for a limited number of antibody combining sitesimmobilized on the well.Unbound conjugate is removed by washing. The enzyme activity in the antibody-bound fraction is inversely proportional to the native total triiodothyronine concentration. Theenzyme activity is revealed by a color change in TMB-substrate solution.

Efficient and Reliable T3 Quantification

Engineered for accuracy and ease of use, the Total T3 ELISA Kit streamlines the process of measuring Total Triiodothyronine in human samples. Featuring high-grade reagents and a user-friendly design, it delivers consistent results within 90 minutes, ideal for both clinical and research settings.

Comprehensive Kit Components

Each kit comes with all necessary materials, including a 96-well polystyrene microplate, standards, conjugate, substrate, stop solution, and wash buffer. Ready-to-use reagents and breakable strips allow flexible, efficient workflow and minimal preparation, maximizing laboratory productivity.

Optimized for Laboratory Performance

The Total T3 ELISA Kit operates manually, requiring no special power or automated equipment. Its robust shelf life (12 months at 2-8C) combined with high sensitivity and specificity ensures reliable testing throughout its use, supporting accurate thyroid function assessment.

FAQs of Total T3 ELISA Kit:

Q: How should the Total T3 ELISA Kit be stored for optimal performance?

A: For best performance and to maintain reagent stability, store the kit and all its components at 2-8C as specified. Avoid repeated freezing and thawing, and ensure that the reagents are brought to room temperature before use for accurate results.

Q: What is the principle behind the Total T3 ELISA Kits detection method?

A: This kit employs a competitive immunoassay format within the Enzyme-Linked Immunosorbent Assay (ELISA) framework. The presence of Total T3 in the sample competes with labeled antigens for binding sites, allowing quantitative measurement via colorimetric change.

Q: When can I expect results after running the Total T3 ELISA assay?

A: The complete assay process can be typically finished in about 90 minutes, enabling rapid turnaround in laboratory settings and providing timely results for clinical decision-making.

Q: Where can the Total T3 ELISA Kit be applied effectively?

A: This kit is intended for in vitro diagnostic use and is well-suited for clinical laboratories, diagnostic centers, and research institutions conducting quantitative analysis of Total T3 in human serum or plasma samples.

Q: What benefits does the kit provide compared to alternative methods?

A: The kit offers high sensitivity and specificity, ready-to-use reagents, and a simple manual process without the need for powered equipment. Its design also includes sealed breakable microplate strips, reducing reagent waste and supporting economical test runs.

Q: How do I use the Total T3 ELISA Kit for testing?

A: Collect human serum or plasma, prepare the samples as instructed, and add them to the provided microplate. Follow the step-by-step protocol for binding, washing, and substrate development, then measure the absorbance to quantify Total T3 concentration within the specified range.

Q: What is included in the Total T3 ELISA Kit package?

A: Each kit contains a 96-well polystyrene microplate, standards, conjugate, substrate, stop solution, wash buffer, and all required high-grade reagents, ensuring all necessary materials are at hand for immediate use.

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