Cytomegalovirus IgM (CMV IgM) ELISA Kit

Cytomegalovirus IgM (CMV IgM) ELISA Kit Specification

Shape
Rectangular microplate

Accuracy
>98%

Features
High Sensitivity and Specificity, Ready-to-use reagents, Colorimetric detection, Easy-to-follow protocol, CE-IVD certified

Temperature Resistance
Store at 2-8C

Control Type
Manual (as per kit protocol)

Type
Immunoassay Kit

Dimension (L*W*H)
Standard kit box, dimensions vary (approx. 30 cm x 25 cm x 10 cm)

Equipment Type
ELISA Kit

Equipment Materials
Plastic, High-Quality Polystyrene Microplate, Buffer Reagents

Material
Plastic (microplate); Reagents in sealed vials

Application
Detection of Cytomegalovirus IgM antibodies in human serum or plasma

Cytomegalovirus IgM (CMV IgM) ELISA Kit Trade Information

Minimum Order Quantity
1 Kit

Sample Policy
If order is confirmed we will reimburse the sample cost

Main Export Market(s)
Middle East, Africa, Asia

Main Domestic Market
All India

About Cytomegalovirus IgM (CMV IgM) ELISA Kit

CMV Ab IgM fast is an one-step sandwich assay based on micro wells coated with recombin ant antigens of Cytomegalo virus (rAg). The conjugate is a mixture of HRP-labeled polyclonal anti-human-IgM antibodies (mAb). Serum samples are added to the wells and if anti-Cytomegalo virus antibodies are present in a sample, they form stable complexes with antigens immobilized on the wells. Then the antigen-antibody complexes are identified by the addition of HRP labeled anti-human- IgM conjugate. The unbound components are removed by washing. After addition of the solution containing TMB and hydrogen peroxide, the wells with bound conjugate develop a blue colour which is converted to yellow after the reaction has been stopped with sulphuric acid. The colour intensity is directly proportional to the concentration of IgM antibodies to Cytomega- lovirus in the specimen and can be read at 450 nm.

Simple and Reliable Testing

Designed with usability in mind, the CMV IgM ELISA Kit includes all essential reagents in ready-to-use format, a high-quality polystyrene microplate, and comprehensive instructions. Breakable strips allow for flexible testing volumes, making it suitable for varying laboratory needs. Colorimetric detection at 450 nm ensures clear and accurate results.

High Sensitivity and Specificity

Benefit from 99% sensitivity and 98% specificity with this kit, greatly reducing the chances of false positives or negatives. The kit has been rigorously validated to provide dependable results for Cytomegalovirus IgM antibody detection, supporting confident clinical decisions.

Efficient and Versatile Application

Each kit provides capacity for 96 tests with an incubation time of about 90 minutes. Its application in detecting CMV IgM antibodies in human serum or plasma makes it a valuable tool for screening and diagnostic purposes. All reagents maintain stability when stored between 28C, with a shelf life of up to 18 months.

FAQs of Cytomegalovirus IgM (CMV IgM) ELISA Kit:

Q: How is the CMV IgM ELISA Kit used to detect Cytomegalovirus IgM antibodies?

A: The kit applies the indirect ELISA method, using an enzyme-labeled antigen and a colorimetric substrate to detect anti-CMV IgM antibodies in human serum or plasma. The presence of these antibodies is determined by measuring the optical density (OD) at 450 nm after adding a substrate to the microplate.

Q: What sample types can be tested with this ELISA kit?

A: This kit is validated for use with human serum or plasma samples, ensuring broad compatibility with standard clinical specimen collections.

Q: When should I interpret the results, and how are they determined?

A: Results are interpreted after an approximately 90-minute incubation period. They are categorized as Positive, Negative, or Borderline based on the samples optical density (OD) value relative to provided controls.

Q: Where should I store the kit and its reagents?

A: To maintain reagent stability and performance, store all components at 28C and protect them from light. Do not freeze the reagents.

Q: What is the process for running the assay?

A: Follow the stepwise protocol included with the kit: add samples and controls to the microplate wells, incubate, wash, add conjugate and substrate, incubate again, then add stop solution before reading the OD value. All steps are described in detail in the instruction manual.

Q: What are the key benefits of using this CMV IgM ELISA Kit?

A: Key benefits include high sensitivity (99%) and specificity (98%), ready-to-use reagents, a straightforward colorimetric detection process, breakable strips for flexible testing, and CE-IVD certification for clinical reliability. The kits accuracy exceeds 98%, ensuring trustworthy results.

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